Calculated binding affinities (Kd) from experimental data that contains dose and response information. Could be titrated concentration and resulting fluorescence, or percentage shift in a EMSA or something similar.
bio_binding(data, conc, resp, group = NULL, slope = 1)data.frame or tibble containing dose response data.
Column with the concentration information.
Column containing the response information.
allowed to be free (set slope = NA).
For all 1:1 binding interactions, the slope for the log - logistic curve should be 1. If the interaction has some cooperativity or it is not a 1:1 binding interaction, then this value will change and should be
a tibble
Puromycin %>%
bio_binding(conc, rate, group = state)
#> # A tibble: 2 × 4
#> state data model coefs
#> <fct> <list> <list> <list>
#> 1 treated <tibble [12 × 2]> <drc> <tibble [3 × 5]>
#> 2 untreated <tibble [11 × 2]> <drc> <tibble [3 × 5]>